CMC0012

XLDNA SIG10 Electrocompetent cells

for BAC & cosmid cloning

• UNSPSC Code: 12352200
• NACRES: NA.85

Properties

• biological source: Escherichia coli
• grade: for molecular biology
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: competent cell type: electrocompetent; transformation efficiency: ≥1 x 10¹⁰ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

The XLDNA SIG10 Electrocompetent cells are designed for transformation of large constructs and increase in DNA yields.
These cells are manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts and are an excellent choice for large construct transformation

Genotype

F - pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 F80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)

Features and Benefits

• Cells that enable the most challenging library construction and screening.
• Highest available transformation efficiencies for library construction and screening.
• Optimized for high yields of DNA

Components

• XLDNA SIG10 electrocompetent cells
• pUC 19 transformation control DNA
• recovery medium for cloning

Safety Information

• Storage Class Code: 10 - Combustible liquids