Skip to Content
Merck
All Photos(3)

Key Documents

Safety Information

C6158

Sigma-Aldrich

Crystal Violet

≥90% purity anhydrous basis, ACS reagent, powder

Synonym(s):

Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B

Sign Into View Organizational & Contract Pricing

Select a Size

50 G
¥13,300
100 G
¥23,900

¥13,300


In StockDetails


Request a Bulk Order

Select a Size

Change View
50 G
¥13,300
100 G
¥23,900

About This Item

Linear Formula:
C25H30N3Cl
CAS Number:
Molecular Weight:
407.98
Colour Index Number:
42555
Beilstein:
3580948
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

¥13,300


In StockDetails


Request a Bulk Order

Product Name

Crystal Violet, ACS reagent, ≥90.0% anhydrous basis

grade

ACS reagent

Quality Level

Assay

≥90.0% anhydrous basis

form

powder

loss

≤7.5% loss on drying

color

green to very dark green

pH

2.5-3.5 (20 °C, 10 g/L)

mp

205 °C (dec.) (lit.)

solubility

water: soluble 50 g/L at 27 °C

density

1.19 g/cm3 at 20 °C

absorption

passes test

suitability

passes test for sensitivity as indicator

antibiotic activity spectrum

fungi

application(s)

diagnostic assay manufacturing
hematology
histology

Mode of action

cell membrane | interferes
enzyme | inhibits

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Looking for similar products? Visit Product Comparison Guide

Application

Crystal violet has been used to determine cell viability.[1] It has been used in the staining of cells for the quantification of cellular migration.[2]

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA.[3] Crystal violet is also used for the staining of bacteria in gram staining technique.[4] It is also used for the staining of plant chromosomes.[5] Crystal violet helps in colorimetric measurement of cell viability.[6]

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

C6158-500G:
C6158-VAR:
C6158-250G:
C6158-50G:
C6158-BULK-9:
2530065:
C6158-BULK:
C6158-100G:


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

The activation of c-Jun NH2-terminal kinase is required for dihydroartemisinin-induced autophagy in pancreatic cancer cells.
Jia G, et al.
Journal of Experimental & Clinical Cancer Research, 33, 8-8 (2014)
Plant Chromosomes (1999)
Downregulation of survivin expression exerts antitumoral effects on mouse breast cancer cells in vitro and in vivo.
Ma WH, et al.
Oncology Letters, 11, 159-159 (2016)
Eric H Clifton et al.
PloS one, 13(3), e0194815-e0194815 (2018-03-23)
Terrestrial plants can harbor endophytic fungi that may induce changes in plant physiology that in turn affect interactions with herbivorous insects. We evaluated whether the application of entomopathogenic fungi Beauveria bassiana and Metarhizium brunneum to soybean seeds could become endophytic
A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet.
Ishiyama M, et al.
Biological & Pharmaceutical Bulletin, 19, 1518-1518 (1996)

Questions

  1. can we make the solution just by dissolving in water?

    1 answer
    1. Yes, this product is soluble in water at 50 mg/mL at 20 °C.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service