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B4527

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

chromogenic, ≥97%, powder

Synonym(s):

X-Glc, X-glucoside

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5 MG
¥20,400
25 MG
¥66,600
100 MG
¥192,000

¥20,400

Estimated to ship onApril 13, 2025

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5 MG
¥20,400
25 MG
¥66,600
100 MG
¥192,000

About This Item

Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
15548-60-4
Molecular Weight:
408.63
Beilstein:
1552603
EC Number:
239-603-0
MDL number:
MFCD00063690
UNSPSC Code:
12352204
PubChem Substance ID:
24891784
NACRES:
NA.83

¥20,400

Estimated to ship onApril 13, 2025

Product Name

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside, ≥97%

Quality Level

200

Assay

≥97%

form

powder

solubility

DMF: 50 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11-,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-LNNRFACYSA-N

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Substrates

A histochemical substrate for β-glucosidase.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

B4527-25MG:
B4527-BULK:
B4527-5MG:
B4527-100MG:
B4527-1MG:
B4527-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
0000332818
0000332818
0000287935
0000287958
SLCQ6536

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L A Shelef et al.
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W D Watkins et al.
Applied and environmental microbiology, 54(7), 1874-1875 (1988-07-01)
A new chromogenic compound, 5-bromo-4-chloro-3-indoxyl-beta-D-glucuronide, was found to be useful for the rapid, specific, differential identification of Escherichia coli in the sanitary analysis of shellfish and wastewater. Of 1,025 presumptively positive colonies (blue) and 583 presumptively negative colonies (nonblue), only
R Gossrau et al.
Histochemistry, 86(4), 397-404 (1987-01-01)
5-Br-4-Cl-3-Indoxyl-alpha-D-gluco(pyrano)side was found to be the most suitable synthetic substrate for the demonstration of alpha-D-glucosidases in situ. Using an azoindoxyl procedure with hexazotized pararosaniline or new fuchsine at pH 5 in freeze-dried celloidine-mounted cryostat sections acid alpha-D-glucosidase (EC 3.2.1.20) was
H Arakawa et al.
Analytical biochemistry, 199(2), 238-242 (1991-12-01)
Chemiluminescent assays of various enzymes have been developed using indoxyl derivatives as substrates. The principle of the method is as follows: an enzyme causes hydrolysis of an indoxyl derivative to an intermediate indoxyl that is readily oxidized to indigo dye
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