MAK461

pNPP Phosphatase Assay Kit

Sufficient for 500 colorimetric tests

• Synonym(s): Phosphatase Assay Kit, p-Nitrophenyl Phosphate Assay Kit
• NACRES: NA.84
• UNSPSC Code: 12161503

Properties

• Quality Segment: 100
• input: protein
• detection method: colorimetric
• relevant disease(s): cancer; hematological disorder; gastrointestinal diseases; neurological disorders; cardiovascular diseases
• storage temp.: −20°C

Description

General description

Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at A₄₀₅ nm on a spectrophotometer.

Application

The kit is suitable for the quantitative determination of phosphatase activity of protein phosphatases.

The pNPP Phosphatase Assay Kit may be used for:

• Metabolism Research
• Cardiovascular Research
• Neurodegenerative Research

Biochem/physiol Actions

The pNPP Phosphatase Assay kit involves adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37 °C.

Features and Benefits

Simplified Process: Enjoy a hassle-free procedure that can be completed within 30 minutes without wash or reagent transfer steps, saving you time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• pictograms: GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H302 + H332,H314,H341,H373,H411
• pcodes: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• target_organs: Nervous system,Kidney,Liver,Skin
• Storage Class: 8A - Combustible corrosive hazardous materials
• wgk: WGK 3