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83850

Sigma-Aldrich

Ribonucleic acid from Torula utilis

Mr 5000-8000

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About This Item

MDL number:
MFCD00132195
UNSPSC Code:
12352106
NACRES:
NA.51

grade

for molecular biology

form

solid

mol wt

Mr 5000-8000

impurities

≤10% water

solubility

0.1 M acetate buffer pH 5.0: soluble 10 mg/mL

shipped in

wet ice

storage temp.

−20°C

General description

RNA from yeast is used in molecular biology laboratories as a carrier during nucleic acid purifications. When used at 10-20 μg/ml, the tRNA acts as a potent co-precipitant that allows for maximal RNA recovery (when working with limited samples).

Application

Ribonucleic acid from Torula utilis was used as a standard in ribonuclease activity assay of certain ribonuclease family members. It was also used as a standard to determine RNA concentration by orcinol assay.
Suitable for use in nucleic acid purifications.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

83850-100G:
83850-VAR:
83850-BULK:
83850-25G:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Russel J Lander et al.
Biotechnology and bioengineering, 79(7), 776-784 (2002-09-05)
Preparative-scale purification of plasmid DNA has been attempted by diverse methods, including precipitation with solvents, salts, and detergents and chromatography with ion-exchange, reversed-phase, and size-exclusion columns. Chromatographic methods such as hydrophobic interaction chromatography (HIC), reversed phase chromatography (RPC), and size
Debi Ranjan Tripathy et al.
Analytical biochemistry, 437(2), 126-129 (2013-03-19)
The ribonuclease (RNase) activity of ribonucleases has been assayed by observing the change in fluorescence intensity of ethidium bromide on binding with yeast RNA. The binding of EtBr with RNA was monitored via UV-vis and fluorimetric methods. The degradation of

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