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R7876

Sigma-Aldrich

Ribonucleic acid, transfer from wheat germ

Type V, 15-19 units/mg solid

Synonym(s):

Transfer RNA, tRNA

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About This Item

CAS Number:
9014-25-9
MDL number:
MFCD00132200
UNSPSC Code:
12352106
NACRES:
NA.55

grade

for molecular biology

Quality Level

300

type

Type V

form

lyophilized powder

specific activity

15-19 units/mg solid

storage condition

desiccated

solubility

H2O: ~2 mg/mL, clear to hazy

storage temp.

2-8°C

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General description

Wheat germ tRNA contains many different specific tRNA molecules.

Application

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.

Components

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Unit Definition

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

R7876-500UN-PW:
R7876-500UN:
R7876-15KU:
R7876-VAR:
R7876-2.5KU:
R7876-10KU:
R7876-BULK:
R7876-10KU-PW:
R7876-2.5KU-PW:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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