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08715

Sigma-Aldrich

DAR-2

BioReagent, suitable for fluorescence, ≥97.0% (HPCE)

Synonym(s):

5,6-Diamino-N,N,N′,N′-tetraethylrhodamine, 5,6-Diamino-rhodamine B

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About This Item

Empirical Formula (Hill Notation):
C28H32N4O3
CAS Number:
Molecular Weight:
472.58
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.32

product line

BioReagent

Assay

≥97.0% (HPCE)

form

powder

fluorescence

λex 562 nm; λem 579 nm in 0.1 M phosphate pH 7.4, NO

suitability

suitable for fluorescence

SMILES string

CCN(CC)c1ccc2c(Oc3cc(ccc3C24OC(=O)c5cc(N)c(N)cc45)N(CC)CC)c1

InChI

1S/C28H32N4O3/c1-5-31(6-2)17-9-11-20-25(13-17)34-26-14-18(32(7-3)8-4)10-12-21(26)28(20)22-16-24(30)23(29)15-19(22)27(33)35-28/h9-16H,5-8,29-30H2,1-4H3

InChI key

LJSMMWFTVBPRDS-UHFFFAOYSA-N

Application

Suitable as fluorescent NO-sensor.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

08715-5MG-F:
08715-BULK-F:
08715-VAR-F:
08715-1MG-F:


Certificates of Analysis (COA)

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H Kojima et al.
Analytical chemistry, 73(9), 1967-1973 (2001-05-17)
Diaminofluoresceins are widely used for detection and imaging of nitric oxide (NO), but for biological applications, they have the disadvantages that the fluorescence of the fluorescein chromophore is pH-sensitive and overlaps the autofluorescence of cells. We have developed a membrane-permeable
H. Kojima et al.
Tetrahedron Letters, 41, 69-69 (2000)

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