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251505-M

Sigma-Aldrich

DAF-2 DA

A cell-permeable derivative of DAF-2 that is hydrolyzed to DAF-2 by intracellular esterases.

Synonym(s):

DAF-2 DA, 4,5-Diaminofluorescein Diacetate

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About This Item

Empirical Formula (Hill Notation):
C24H18N2O7
CAS Number:
205391-02-2
Molecular Weight:
446.41
MDL number:
MFCD05865101
UNSPSC Code:
12171500
NACRES:
NA.25

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Quality Level

100

Assay

≥98% (HPLC)

form

solution

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

pale yellow

fluorescence

λex ~495 nm
λem ~515 nm

storage temp.

−20°C

SMILES string

Nc1cc2c(cc1N)C(=O)OC32c4c(cc(cc4)OC(=O)C)Oc5c3ccc(c5)OC(=O)C

InChI

1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3

InChI key

PTSUYDXEEKDBQU-UHFFFAOYSA-N

General description

A cell-permeable derivative of DAF-2 (Cat. No. 251500) that is hydrolyzed to DAF-2 by intracellular esterases. Can be used in fluorescence microscopy to measure real-time changes in NO levels in vivo.

Biochem/physiol Actions

Primary Target
Used to measure real-time changes in NO levels

Warning

Toxicity: Irritant (B)

Physical form

A 5 mM (1 mg/450 µl) solution in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.

Legal Information

Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F - closed cup - (Dimethylsulfoxide)

Flash Point(C)

87 °C - closed cup - (Dimethylsulfoxide)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

FSL

Group 4: Flammable liquids
Type 3 petroleums
Hazardous rank III
Water insoluble liquid

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

251505-1MG:
251505-MG:
251505-1MG-M:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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