Activation is not required for this preparation. However, the beads should be washed thoroughly prior to use y with 5-10 column volumes of buffer, to remove the sodium azide in the storage buffer. Please see the link below to review the datasheet for product S1638, this may be applied to this material.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/424/273/s1638pis-ms.pdf
85881
Streptavidin, immobilized on Agarose CL-4B
suitable for affinity chromatography
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1 ML
¥34,200
5 ML
¥133,000
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1 ML
¥34,200
5 ML
¥133,000
About This Item
UNSPSC Code:
12190000
NACRES:
NA.21
Recommended Products
form
suspension
Quality Level
contains
0.02% NaN3 as preservative
technique(s)
affinity chromatography: suitable
capacity
≥15 μg/mL, packed gel capacity (biotin)
storage temp.
2-8°C
General description
streptavidin attached to 4% beaded cross-linked agarose via a C6-spacer (1.2 mg streptavidin/ml packed gel)
Physical form
50% suspension in 0.01 M sodium phosphate buffer, pH 7.2, containing 0.15 M sodium chloride
Other Notes
Isolation of cell surface glycoprotein by biotinylated concanavalin A and immobilized streptavidin[1].
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
85881-VAR:
85881-1ML:
85881-5ML:
85881-BULK:
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I want to know if before using the beads I need activate it with any specific solution. Could you help me please.
1 answer-
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