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248266

Sigma-Aldrich

Xylidyl blue I

synthetic colorimetric reagent

Synonym(s):

4-Hydroxy-3-[2-hydroxy-3-(2,4-dimethylphenylaminocarbonyl)-1-naphthylazobenzenesulfonic acid sodium salt, Magonsulfonate

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About This Item

Linear Formula:
HOC6H3[N=NC10H5(OH)[CONHC6H3(CH3)2]]SO3Na
CAS Number:
14936-97-1
Molecular Weight:
513.50
Beilstein:
4107363
EC Number:
239-012-8
MDL number:
MFCD00003890
UNSPSC Code:
12171500
PubChem Substance ID:
24854925
NACRES:
NA.47

Quality Level

200

form

powder or crystals

technique(s)

titration: suitable

color

dark violet

λmax

612 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Cc1ccc(NC(=O)c2cc3ccccc3c(\N=N\c4cc(ccc4O)S([O-])(=O)=O)c2O)c(C)c1

InChI

1S/C25H21N3O6S.Na/c1-14-7-9-20(15(2)11-14)26-25(31)19-12-16-5-3-4-6-18(16)23(24(19)30)28-27-21-13-17(35(32,33)34)8-10-22(21)29;/h3-13,29-30H,1-2H3,(H,26,31)(H,32,33,34);/q;+1/p-1/b28-27+;

InChI key

LKSHJHJGLORTGD-RXQWRGDBSA-M

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General description

Xylydil Blue I is a synthetic colorimetric reagent that has been widely used for the spectrophotometric detection of magnesium.

Application

Xylydil Blue I is suitable to determine serum magnesium levels using Mg- Xylydil Blue I complex based method.

Biochem/physiol Actions

Xylydil Blue I forms a purple complex with the magnesium ion in alkaline conditions.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

248266-VAR:
248266-5G:
248266-1G:
248266-BULK:
248266-10G:

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM1527
BCCL3466
BCCJ8558
BCCK8058
BCCG9053

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M J Stone et al.
Clinical chemistry, 42(9), 1474-1477 (1996-09-01)
We have evaluated an enzymatic assay for the determination of Mg in serum, based on the activation of a chemically modified form of isocitrate dehydrogenase by Mg2+. In the presence of potassium isocitrate and NADP+, NADPH, which absorbs light at
Measurement of magnesium in serum and urine with a random access analyzer by use of a modified xylidyl blue-1 procedure.
D Ratge et al.
Clinica chimica acta; international journal of clinical chemistry, 159(2), 197-203 (1986-09-15)
[Measurement of magnesium in the human sera with xylidyl-blue I. I. The effect of phosphate ion].
Y Takubo et al.
Rinsho byori. The Japanese journal of clinical pathology, 31(11), 1244-1248 (1983-11-01)
Determination of urine magnesium using the magon dye method on the 'Monarch' centrifugal analyser.
W Davidson et al.
Annals of clinical biochemistry, 27 ( Pt 6), 595-596 (1990-11-01)
M R Wills et al.
Magnesium, 5(5-6), 317-327 (1986-01-01)
A variety of methods have been reported for the quantitative analysis of magnesium in serum and other biological fluids. The methods may be classified into the following groups: magnesium ammonium phosphate; 8-hydroxyquinoline; titan yellow; dihydroxyazobenzene using fluorimetry; Schwarzenbach ethylenediaminetetraacetic acid;

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