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SCC048

Sigma-Aldrich

N27 Rat Dopaminergic Neural Cell Line

Rat

Synonym(s):

Dopamine Neural Cells, 1RB3AN27 Cell Line

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81

product name

N27 Rat Dopaminergic Neural Cell Line, The N27 rat dopaminergic neural cell line has been used as a dopaminergic neuron model for in vitro and in vivo studies.

biological source

rat

Quality Level

100

technique(s)

cell culture | stem cell: suitable

shipped in

liquid nitrogen

General description

Parkinson’s disease is a neurodegenerative disorder of the central nervous system that affects more than 6 million people worldwide. The motor symptoms of Parkinson′s disease result from the death of dopamine generating cells in the substantia nigra, a region of the midbrain. Numerous efforts have been made to slow the loss or replace these dopamine producing neurons in vivo.
The N27 rat dopaminergic neuron cell line was harvested from E12 rat mesencephalic tissue and was transfected with SV40 to immortalize the cell line. The N27 cell line, when injected into the striata of 6-hydroxydopamine-lesioned rats (an animal model of PD) caused a time-dependent improvement in neurological deficits. This immortalized cell line has been carefully characterized in studies of dopamine biosynthesis, neurotoxicity and used as a dopaminergic neuron model for in vitro and in vivo studies.

Cell Line Description

Neural Stem Cells

Neural Lineage Cells

Application

Research Category
Stem Cell Research

Neuroscience
Research Sub Category
Neural Stem Cells

Neurochemistry & Neurotrophins

Neurodegenerative Diseases

Neuroregenerative Medicine
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.

Quality

• Each vial contains ≥ 1X106 viable cells.
• Cells are negative for mycoplasma contamination.

Storage and Stability

N27 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

SCC048:

Certificates of Analysis (COA)

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Fatema Bhinderwala et al.
Methods in molecular biology (Clifton, N.J.), 1996, 217-257 (2019-05-28)
Metabolomics has been successfully applied to study neurological and neurodegenerative disorders including Parkinson's disease for (1) the identification of potential biomarkers of onset and disease progression; (2) the identification of novel mechanisms of disease progression; and (3) the assessment of
Ryan P McGlinchey et al.
The Journal of biological chemistry, 294(25), 9973-9984 (2019-05-17)
A pathological feature of Parkinson's disease (PD) is Lewy bodies (LBs) composed of α-synuclein (α-syn) amyloid fibrils. α-Syn is a 140 amino acids-long protein, but truncated α-syn is enriched in LBs. The proteolytic processes that generate these truncations are not
Establishment and characterization of immortalized clonal cell lines from fetal rat mesencephalic tissue.
Prasad, K N, et al.
In Vitro Cellular & Developmental Biology. Animal, 30A, 596-603 (1994)
PKC? mediates paraquat-induced Nox1 expression in dopaminergic neurons.
Cristov?o, Ana Clara, et al.
Biochemical and biophysical research communications, 437, 380-385 (2013)
Characterization and transplantation of two neuronal cell lines with dopaminergic properties.
Adams, F S, et al.
Neurochemical Research, 21, 619-627 (1996)
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