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D6046

Sigma-Aldrich

DMEM - low glucose

With sodium bicarbonate, sodium pyruvate and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

MDL number:
MFCD00217342
UNSPSC Code:
12352207
NACRES:
NA.75

product name

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: yes
sodium pyruvate: yes
phenol red: yes
NaHCO3: yes
HEPES: no
glucose: low

shipped in

ambient

storage temp.

2-8°C

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General description

This DMEM-Low glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Application

Dulbecco′s Modified Eagle′s Medium-low glucose is a fully supplemented liquid form of the original Dulbecco′s formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.
Dulbecco′s Modified Eagle′s Medium - low glucose has been used in the maintenance/culturing of mammalian cells.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

D6046-BULK:
D6046-6X1L:
D6046-PH:
D6046-1L:
D6046-VAR:
D6046-6X500ML:
D6046-100ML:
D6046-500ML:
D6046PROC:
D6046-24X500ML:

Certificates of Analysis (COA)

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Nicotine increases cancer stem cell population in MCF-7 cells.
Hirata N et al.
Biochemical and Biophysical Research Communications, 403, 138-138 (2010)
Nuclear receptor liver X receptor is O-GlcNAc-modified in response to glucose.
Anthonisen EH et al.
The Journal of Biological Chemistry, 285, 1607-1607 (2010)
High glucose-induced mitochondrial respiration and reactive oxygen species in mouse cerebral pericytes is reversed by pharmacological inhibition of mitochondrial carbonic anhydrases: Implications for cerebral microvascular disease in diabetes.
Shah GN et al.
Biochemical and Biophysical Research Communications, 440, 354-354 (2013)
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PLoS computational biology, 13(12), e1005913-e1005913 (2017-12-28)
Cells decode information of signaling activation at a scale of tens of minutes by downstream gene expression with a scale of hours to days, leading to cell fate decisions such as cell differentiation. However, no system identification method with such
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