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MAB1021

Sigma-Aldrich

Anti-Tumor Necrosis Factor-α Antibody, clone 2C8

clone 2C8, Chemicon®, from mouse

Synonym(s):

TNF-alpha

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

clone

2C8, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
neutralization: suitable

isotype

IgG1

NCBI accession no.

NM_000594.2

UniProt accession no.

P01375

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TNF(7124)

Specificity

Human tumor necrosis factor-a (TNF-a)
No cross-reactivity observed with rat neuronal tissue.

Immunogen

Human recombinant tumor necrosis factor α.

Application

Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody, clone 2C8 validated for use in ELISA, IH, NEUT.
Inhibition Assays – Inhibition of biological activity of recombinant and natural TNF-α.
Detection of TNF-α in two-site immunoassay.

Immunohistochemistry detected TNF-α.

TNF-α Neutralization Assay

1. Plate 2 x 104 murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate.

2. Incubate overnight at 37°C in humidified 5% CO2 incubator.

3. The next day, prepare serial dilutions of anti-TNF (human) in medium containing 2 μg/mL of actinomycin D, for a final volume of 50 μL

4. Add 50 μL of TNF (10 ng/mL final concentration) to each antibody dilution and incubate for 1 hour at 37°C.

5. Add 100 μL of the antibody/antigen mixture to the pre-seeded L929B cells.

6. Incubate 24 hours at 37°C, 5% CO2.

7. Score wells for cytotoxicity.

Optimal working dilutions must be determined by the end user.

Linkage

Replaces: 04-1114

Physical form

Format: Purified

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to one year.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAB1021-KC:
MAB1021:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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