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HTS153RTA

Sigma-Aldrich

Ready-to-Assay GPR68/OGR1 Proton-Sensing Receptor Frozen Cells

Human

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.84

product name

Ready-to-Assay GPR68/OGR1 Proton-Sensing Receptor Frozen Cells, Human GPR68/OGR1 GPCR frozen cells for Calcium Flux FLIPR Assays.

biological source

human

Quality Level

100

manufacturer/tradename

Ready-to-Assay

technique(s)

calcium flux assay: suitable

NCBI accession no.

NM_003485

detection method

fluorometric

shipped in

dry ice

General description

Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

GPR68, also known as OGR1 (ovarian cancer G-protein-coupled receptor 1), was initially thought to be a receptor for sphingosylphosphorylcholine, although these results are controversial. Subsequent studies indicated that GPR68/OGR1 functions as a Gq-coupled sensor for extracellular pH, with maximal signaling occurring at pH of 7 or below (Ludwig et al., 2003; Tomura et al., 2005). Expression of GPR68/OGR1 is upregulated during RANKL-induced osteoclast differentiation, and knockdown of GPR68/OGR1 expression inhibits this differentiation process (Yang et al., 2006). Millipore’s cloned human GPR68-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GPR68 expression on the cell surface and contains high levels of the promiscuous G protein G15 to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between GPR68 and its ligands.

Cell Line Description

GPCR Cell Lines
Host cells: Chem-1

Biochem/physiol Actions

GPCR Class: A
Protein Target: GPR68/OGR1
Target Sub-Family: SPC/LPC /proton-sensor

Components

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.

Fifty (50) mL of Media Component.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

HTS153RTA:

Certificates of Analysis (COA)

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