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HTS102RTA

Sigma-Aldrich

Ready-to-Assay D 1 Dopamine Receptor Frozen Cells

Human

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.76

product name

Ready-to-Assay D 1 Dopamine Receptor Frozen Cells,

biological source

human

Quality Level

100

manufacturer/tradename

Ready-to-Assay

technique(s)

calcium flux assay: suitable

NCBI accession no.

NM_000794

detection method

fluorometric

General description

Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Dopamine exerts its action by binding to five distinct dopamine receptors, all of which belong to G protein-coupled receptor family (Missale et al. 1998). The D1 subtype is the most abundant dopamine receptor in the central nervous system. Activation of D1 receptor stimulates adenylyl cyclase, activates cyclic AMP-dependent protein kinases. It regulates neuronal growth and development, mediate some behavioral responses and modulate dopamine receptor D2-mediated events. Millipore′s cloned human D1-expressing cell line is made in the Chem-6 host, which supports high levels of recombinant D1 expression and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at D1.

Cell Line Description

  • GPCR Cell Lines
  • Host cells: Chem-6

Application

Ready-to-Assay Human D1 GPCR frozen cells for Calcium Flux FLIPR Assays.

Biochem/physiol Actions

  • GPCR Class: A
  • Protein Target: D1
  • Target Sub-Family: Dopamine

Components

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.

Disclaimer


This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

HTS102RTA:

Certificates of Analysis (COA)

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