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HTS082RTA

Sigma-Aldrich

Ready-to-Assay 5-HT 2A Serotonin Family Receptor Frozen Cells

Human

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81

product name

Ready-to-Assay 5-HT 2A Serotonin Family Receptor Frozen Cells,

biological source

human

Quality Level

100

manufacturer/tradename

Ready-to-Assay

technique(s)

calcium flux assay: suitable

NCBI accession no.

NM_000621

detection method

fluorometric

General description

Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5-HT2A, which couples to Gq/11 to increase intracellular calcium, is widely expressed at central and peripheral sites of 5-HT action, and contributes to many of the physiological effects of 5-HT. The hallucinogenic activity of LSD is mediated in part by its action as an partial to full agonist at 5-HT2A, and the activity of atypical antipsychotics such as clozapine appears to be mediated in part by antagonism of 5-HT2A (Barnes and Sharp, 1999). Millipore′s cloned human 5-HT2A -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant 5-HT2A expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at 5-HT2A.

Cell Line Description

  • GPCR Cell Lines
  • Host cells: Chem-1

Application

Human 5-HT2A GPCR frozen cells for Calcium Flux FLIPR Assays.

Biochem/physiol Actions

  • GPCR Class: A
  • Protein Target: 5-HT2A
  • Target Sub-Family: Serotonin

Components

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.

Disclaimer


This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

HTS082RTA:

Certificates of Analysis (COA)

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