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ABD43

Sigma-Aldrich

Anti-Apelin receptor Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Apelin Receptor, Angiotensin Receptor-Like 1, G-Protein Coupled Receptor Apj, G-Protein Coupled Receptor Hg11

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

species reactivity (predicted by homology)

rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

Np_005152

UniProt accession no.

P35414

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... APLNR(187)

General description

The Apelin receptor (APJ receptor) is a Gi/o-coupled protein with significant sequence homology to the angiotensin II type 1 (AT1) receptor. It is activated by apelin peptides (12, 17, or 36 amino acids). The effects of the apelin receptor are mediated mainly through the activation of protein kinase C and calcium signaling. The apelin receptor has been detected in the brain, heart, spleen, thymus, ovary, small intestine, and colon. Several studies have suggested that it is involved in regulating cardiovascular function and glucose homeostasis.

Specificity

This antibody recognizes Apelin receptor at the cytoplasmic domain.

Immunogen

Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Apelin receptor.

Application

Research Category
Stem Cell Research
This Anti-Apelin receptor Antibody is validated for use in Western Blotting, IHC(P) for the detection of Apelin receptor.
Western Blot Analysis: 0.5 µg/mL from a representative lot detected Apelin receptor on 10 µg of human cerebellum tissue lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Apelin receptor in several regions of mouse brain sections.

Quality

Evaluated by Western Blot in mouse cerebellum tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Apelin receptor on 10 µg of mouse cerebellum tissue lysate.

Target description

~62 kDa observed. The calculated molecular weight of this protein is 42 kDa, but can be observed at ~62 kDa due to high glycosylation. (Medhurst, A., et al (2003). Journal of Neurochemistry. 84:1162–1172.)

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse Cerebellum Tissue Lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

ABD43:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Milirani Das et al.
Journal of experimental zoology. Part A, Ecological and integrative physiology, 341(4), 450-457 (2024-02-23)
The apelin receptor (APJ) belongs to the member of the G protein-coupled receptor family, and expression of APJ has been reported in the different cell types of testis. The seminiferous tubules in the testis can be identified as different stages
Ravinder Reddy Gaddam et al.
Clinical and translational medicine, 12(1), e693-e693 (2022-01-22)
MicroRNAs regulate cardiac hypertrophy development, which precedes and predicts the risk of heart failure. microRNA-204-5p (miR-204) is well expressed in cardiomyocytes, but its role in developing cardiac hypertrophy and cardiac dysfunction (CH/CD) remains poorly understood. We performed RNA-sequencing, echocardiographic, and
Andrea David Re Cecconi et al.
Cancers, 14(7) (2022-04-13)
Cancer cachexia consists of dramatic body weight loss with rapid muscle depletion due to imbalanced protein homeostasis. We found that the mRNA levels of apelin decrease in muscles from cachectic hepatoma-bearing rats and three mouse models of cachexia. Furthermore, apelin
Ayushmita Dutta et al.
Cell biology international (2024-04-18)
Apelin and its receptor (APJ) are expressed in the reproductive organs of some mammalian females. The function of oviduct has also been suggested to be compromised in the hyperandrogenism condition. However, expression of apelin and APJ has not been shown
Sema Avci et al.
The International journal of developmental biology, 67(3), 91-100 (2023-11-08)
Although preterm birth is among the preventable causes of maternal and infant death, its mechanism has not yet been clarified. When evaluated in terms of the results, the psycho-social burden of mother-infant losses and the costs of rehabilitation, care, and
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