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AB1589P

Sigma-Aldrich

Anti-Adenosine A2b Receptor Antibody

Chemicon®, from rabbit

Synonym(s):

A2b Receptor Antibody, Adenosine A2b Antibody, Anti-Adenosine Receptor

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_000676.2

UniProt accession no.

P29275

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ADORA2B(136)

Specificity

Adenosine A2b receptor. Recognizes a band of approximately 50-52 kDa in several rat, mouse and human tissues (thymus, placenta, colon and small intestine). The antibody also recognizes the 35 kDa band in small intestine. No bands were detected in skeletal muscle or heart.

Immunogen

Amino acid sequence corresponding to the 2nd extracellular domain (16 amino acids) of the human brain A2bR gene (Pierce et al. 1992; Tucker & Linden 1993).

Application

Detect Adenosine A2b Receptor using this Anti-Adenosine A2b Receptor Antibody validated for use in ELISA, IC, IH & WB.
Immunohistochemistry: 10-50 μg/mL on frozen and formalin fixed tissue sections. The receptor was localized in epithelial cells of the crypts, and in synctiotrophoplast cells of human placenta and in T84 human colon adenocarcinoma cells.

Western blot: 1-10 μg/mL (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.

Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.

ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG292)/well)

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Circadian Rhythm & Sleep

Physical form

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB1589P:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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