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72620-M

Sigma-Aldrich

NovaQUANT® Human Mitochondrial to Nuclear DNA Ratio Kit

qPCR format, for human DNA sample extracted from cells or tissues of interest

Synonym(s):

Human mtDNA-to-nDNA ratio kit

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About This Item

UNSPSC Code:
41105500

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

RT-PCR: suitable

storage temp.

−20°C

General description

The NovaQUANT® Human Mitochondrial to Nuclear DNA Ratio Kit compares the levels of nuclear to mitochondrial DNA (mtDNA) in a human DNA sample.

Real-Time PCR provides a platform for a quantitative assay measuring and comparing mtDNA copy number to that of nuclear DNA (nDNA). This mtDNA/nDNA ratio test kit in qPCR format can be used whenever a small amount of DNA can be extracted from cells or tissues of interest. The primers in this kit have been thoroughly validated for matching amplification efficiency and for absence of amplification of mitochondrial pseudogenes.

Application

A single assay is run using four wells, whereby each well individually tests for one of four gene targets. Resultant Cts obtained from the Real-Time instrument are used to represent the level of each gene. Nuclear gene 1 is compared to that of mitochondrial gene 1 and nuclear gene 2 to mitochondrial gene 2. With this method, ratios are calculated which can be averaged to represent the mtDNA copy number per cell.

This kit contains a set of four optimized PCR primer pairs targeting two nuclear and two mitochondrial genes.

Human Panel
Mitochondrial Genes: ND1,ND6
Nuclear DNA: BECN1, NEB1

Features and Benefits

NovaQUANT assays accelerate your research

  • Matched amplification efficiencies: Optimizes mitochondrial and nuclear primer pairs for accurate determination of DNA ratios (based on primer pair amplification efficiencies)
  • Validated gene targets: Eliminates detection of mitochondrial pseudogenes
  • Accurate gene expression detection: qPCR protocol optimized for sensitive, specific and linear quantification of mitochondrial or nuclear target genes
  • High specificity standard(s): Validates Control (wild type and mitochondrial negative) total DNA
  • Convenient platform: Plates with pre-aliquoted primers for automating your protocols

Components

  • 2 ea qPCR plate with pre-aliquoted PCR primer pairs
  • 100ng Human wt DNA (1 ng/μl)
  • 100ng Human rho zero DNA (1 ng/μl)

This kit contains a set of four optimized PCR primer pairs targeting two nuclear and two mitochondrial genes.

Warning

Toxicity: Standard Handling (A)

Other Notes

Storage: All materials in this kit can be kept at 4°C for up to three months; we recommend keeping control DNAs at -20 °C for long term storage.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAQUANT is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

72620-1KIT:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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