MITOISO2

Mitochondria Isolation Kit

sufficient for 50 applications (2-5 x 10⁷ cells), isolation of enriched mitochondrial fraction from cells

• Synonym(s): Mitochondrial Extraction Kit, Mitochondrial Purification Kit
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• Quality Level: 200
• usage: sufficient for 50 applications (2-5 x 10⁷ cells)
• technique(s): ELISA: suitable; western blot: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells. The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.

Application

Mitochondria Isolation Kit has been used in the isolation of mitochondria from:

• Ewing′s Sarcoma Family of Tumor (ESFT) cells
• blood samples of tuberculosis patients
• human neuroblastoma cells and glioma cells
• myotubes
• HepG2 cells

Features and Benefits

• The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
• The isolated mitochondria will mostly have intact inner and outer membranes.
• Can be used to extract mitochondrial proteins for proteome analysis

Other Notes

• This product is for R&D use only, not for drug, household, or other uses.
• Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
• Use ultrapure water for the preparation of reagents.
• Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.

Kit Components Also Available Separately

• C2978 CelLytic^™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mL
• P8340 Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mL
• C0356 Protein Extraction Reagent Type 4 1 bottle

Safety Information

• Pictograms: GHS08,GHS07,GHS09
• Signal Word: Warning
• Hazard Statements: H302,H315,H319,H351,H361fd,H411
• Precautionary Statements: P202 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup