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70979

Sigma-Aldrich

BetaFluor β-Galactosidase Assay Kit

Optimized reagents for ultrasensitive β-gal assays

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About This Item

UNSPSC Code:
41106513
NACRES:
NA.54

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze

storage temp.

−20°C

General description

Optimized reagents for ultrasensitive β-gal assays
The BetaFluor β-Gal Assay Kit is designed for rapid, sensitive measurement of β-galactosidase activity in cell extracts. The kit features Reportasol Extraction Buffer, which enables efficient passive extraction of cell protein while maintaining optimal enzyme activity, and is configured for 96-well format. Because no mechanical operations are necessary for extraction, the assay is highly amenable to high-throughput reporter gene expression analysis. The BetaFluor Assay is designed for fluorometric detection with 500-fg sensitivity.

Components

•100 mgBetaFluor Substrate

•25 mlReportasol Extraction Buffer

•100 mlReaction Buffer

•50 mlStop Buffer

•1.6 ml1 M DTT

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H315,H318

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

70979-3:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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