524627

Phosphatase Inhibitor Cocktail III

liquid, for the inhibition of serine/threonine and protein tyrosine phosphatases

• Synonym(s): Phosphatase Inhibitor Mix (Set III), Phosphatase inhibitor
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• product name: Phosphatase Inhibitor Cocktail Set III, Phosphatase Inhibitor Cocktail Set III is a ready to use cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases.
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Phosphorylation and dephosphorylation regulate signal transduction processes involved in cellular activities such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific amino acid residues (serine, threonine, or tyrosine) on target proteins, changing their conformation and interaction with ligands. Protein phosphatases remove the phosphate group, restoring the protein to its dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain their phosphorylation status intact. Phosphatase Inhibitor Cocktail Set III is a cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials.

Application

Phosphatase Inhibitor Cocktail Set III has been used:

• as a supplement in lysis buffer to prepare protein lysates of whole testes for western blotting
• to fortify ice-cold lysis radio-immunoprecipitation assay (RIPA) buffer to homogenize larval hindguts for western blot analysis
• as a component in cell lysis buffer to extract total proteins for western blotting

Biochem/physiol Actions

Cell permeable: no

Primary Target
Serine/threonine and protein tyrosine phosphatases

Product does not compete with ATP.

Reversible: no

Features and Benefits

• Ready-to-use formulations for convenient use
• High-quality products for consistent and reproducible performance,
• Flexible options with a range of cocktails targeting different categories of phosphatases

Components

Each vial contains 1 ml of aqueous solution with the following components:

• 50 mM Sodium Fluoride
• 10 mM ß-Glycerophosphate (Cat. No. 35675)
• 10 mM Sodium Pyrophosphate Decahydrate
• 1 mM Sodium Orthovanadate

Warning

Toxicity: Toxic (F)

Physical form

In H₂O.

Reconstitution

For long-term storage, aliquot and freeze (-20°C). Dilute 1:5 just prior to use.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable