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Key Documents

Safety Information

194932

Sigma-Aldrich

AZOCOLL Substrate, <50 Mesh

General proteolytic substrate.

Synonym(s):

AZOCOLL Substrate, <50 Mesh

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About This Item

CAS Number:
53092-90-3
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

100

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

dark red

solubility

aqueous buffer: soluble
water: soluble

shipped in

ambient

storage temp.

10-30°C

General description

General proteolytic substrate. Provides a rapid and simple spectrophotometric method to detect the presence of proteases in aqueous solutions. As the insoluble substrate is cleaved by proteases, it releases a red dye into the surrounding buffer. Incubation of 50 mg AZOCOLL Substrate with 2 units of PRONASE Protease for 15 min yields an absorbance of greater than 1.0 at 520 nm.
This product has been discontinued.


General proteolytic substrate. Provides a rapid, simple method for the detection of proteases in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 50 mg/5 ml buffer, 10 minutes at 37°C, pH 7.0): <0.2. Mesh: <50 (minimum 80%).

Warning

Toxicity: Standard Handling (A)

Other Notes

Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.
Moore, G.L. 1969. Anal. Biochem. 32, 122.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

194932-1GM:
194932-VGM:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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