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Safety Information

C0528

Sigma-Aldrich

Casein fluorescein isothiocyanate from bovine milk

Type III, essentially salt-free, lyophilized powder

Synonym(s):

FITC-casein

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10 MG
¥28,700
25 MG
¥46,900
100 MG
¥134,000

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10 MG
¥28,700
25 MG
¥46,900
100 MG
¥134,000

About This Item

MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32

¥28,700


Available to ship onMarch 31, 2025Details


Request a Bulk Order

type

Type III

form

essentially salt-free, lyophilized powder

extent of labeling

≥50 μg FITC per mg solid

solubility

water: 5 mg/mL, clear to hazy

storage temp.

2-8°C

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Application

Casein fluorescein isothiocyanate from bovine milk has been used:
  • in accessing zona pellucida (ZP) and perivitelline space (PVS) to evaluate changes induced by protease treatment[1]
  • to make a reference line in immunochromatographic devices[2]
  • in running gel for the documentation of proteinases[3]

highly sensitive protease substrate

Biochem/physiol Actions

Casein fluorescein isothiocyanate is mainly used as a substrate in estimating the protease activity of psychrotrophic bacteria. It is also used as a substrate for determining the activity of bacteria in milk.[4]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C0528-25MG:
C0528-100MG:
C0528-10MG:
C0528-BULK:
C0528-VAR:


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Casein, fluorescein isothiocyanate as a substrate for measuring protease production by psychrotrophic bacteria
Christen GL and Senica M
Journal of Food Protection, 50(9), 744-749 (1987)
Immunochromatographic assay for simple and rapid detection of Satsuma dwarf virus and related viruses using monoclonal antibodies
Kusano N, et al.
Journal of General Plant Pathology, 73(1), 66-71 (2007)
Tomasz Chamera et al.
Journal of molecular biology, 431(11), 2180-2196 (2019-04-27)
Hsp104 is a yeast chaperone that rescues misfolded proteins from aggregates associated with proteotoxic stress and aging. Hsp104 consists of N-terminal domain, regulatory M-domain and two ATPase domains, assembled into a spiral-shaped hexamer. Protein disaggregation involves polypeptide extraction from an
Yong Tang et al.
PLoS biology, 17(2), e3000136-e3000136 (2019-02-07)
Endosymbiosis has driven major molecular and cellular innovations. Plasmodium spp. parasites that cause malaria contain an essential, non-photosynthetic plastid-the apicoplast-which originated from a secondary (eukaryote-eukaryote) endosymbiosis. To discover organellar pathways with evolutionary and biomedical significance, we performed a mutagenesis screen
The supplementation of culture medium with protease improves the hatching rate of mouse embryos
Lee DR, et al.
Human Reproduction, 12(11), 2493-2498 (1997)

Articles

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Questions

  1. What's the mwt of fitc-casein in kda?

    1 answer
    1. The Casein used to prepare this product is a mixture of Alpha-s1, Alpha-s2, Beta, and Kappa Casein. Combined, these forms of casein have a molecular weight range of 19 - 25.2 kDa. The Fluorescein isothiocyanate I isomer has a molecular weight of 389.38. The ratio of casein to FITC is approximately 11:1. A confirmed molecular weight has not been established.

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