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1.09093

Sigma-Aldrich

AFB-Fluor

Staining kit for fluorescence-microscopic detection of acid fast bacteria

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About This Item

UNSPSC Code:
41116130

Quality Level

form

liquid

IVD

for in vitro diagnostic use

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

General description

AFB-Fluor -Staining kit for fluorescence-microscopic detection of acid fast bacteria, is a staining using using fluorescence which allows acid-fast bacteria (AFB) to be easily identified even when their numbers are small. As it has become an established procedure alongside Ziehl-Neelson staining, we now provide a ready-to-use AFB-fluor staining kit. The kit contains three solutions, of which the first solution contains the two fluorescent dyes, Rhodamine B – staining red-orange - and Auramine O – staining yellow-green – which can be used depending on the microscopic filters. Further, the kit supplies a decolorization solution and a potassium permanganate solution that turns the background uniformly black, suppressing troublesome intrinsic fluorescence of the specimen. If required, double staining with AFB-Color modified (Product number 1.00497)(ZN) is possible. AFB-fluor, modified is designed to be used for smears, thougt it can also be used for paraffin sections. The phenol-free kit AFB-fluor phenol-free (Product number 1.01597) can also be used as a user-friendly alternative.
The kit is sufficient for up to 300 applications. It is an IVD product and CE-certified, thus it can be used in diagnostic applications. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy: passes test
Mycobacteria: reddish/greenish
Background: dark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 3


Certificates of Analysis (COA)

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