1.01597

AFB-Fluor phenol-free

Staining kit for the examination of acid-fast bacteria with fluorescence microscopy (Auramin-Rhodamine staining)

• UNSPSC Code: 41116130
• NACRES: NA.74

Properties

• Quality Level: 400
• IVD: for in vitro diagnostic use
• application(s): clinical testing; diagnostic assay manufacturing; hematology; histology
• detection method: fluorometric (microscopy)
• storage temp.: 15-25°C

Description

General description

The decisive feature of the AFB-Fluor phenol-free for detection of aldehyde and mucosubstances, is the presence of fluorescence dyes in the staining solution. This enables the swift and secure identification of the acid-fast bacteria under the fluorescence microscope. As it has become an established procedure alongside Ziehl-Neelson staining (AFB-Color modified), we provide three ready-to-use solutions in our AFB-fluor, phenol-free staining kit. Of these, the first solution contains the two fluorescent dyes, Rhodamine B – staining red - and Auramine O – staining yellowish-green – which can be used depending on the filter combination of the microscope. Here, the auramine-rhodamine staining solution is prepared without phenol and the efficient penetration of the dyes into the target structures is achieved through a phenol substitute that is more user- and eco-friendly than phenol. Further, the kit supplies a decolorization solution and a potassium permanganate solution that turns the background uniformly black, suppressing troublesome intrinsic fluorescence of the specimen. If required, double staining with AFB-Color modified (Product number 1.00497) (ZN) is possible. The AFB-fluor, phenol-free-kit, modified is designed to be used for smears, thougt it can also be used for paraffin sections.
The kit is sufficient for up to 60-65 preparations and is an IVD / CE registered product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy: passes test
Mycobacteria: red-orange/yellow-green
Background: dark

Safety Information

• Pictograms: GHS02,GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H225 - H290 - H319 - H336 - H411
• Precautionary Statements: P210 - P233 - P234 - P240 - P273 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3