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C9422

Sigma-Aldrich

Cellulase from Trichoderma viride

greener alternative

crude powder, 3-10 units/mg solid

Synonym(s):

1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase

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About This Item

CAS Number:
9012-54-8
Enzyme Commission number:
3.2.1.4 (BRENDA, IUBMB)
EC Number:
232-734-4
MDL number:
MFCD00081510
UNSPSC Code:
12352204

form

crude powder

specific activity

3-10 units/mg solid

greener alternative product characteristics

Waste Prevention: Greener alternative product characteristics
Learn more about the Principles of Green Chemistry.

greener alternative category

Enabling

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.

Application

1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase, or cellulase, has been used in a study to examine supra-molecular structural features of cellulose during enzymatic hydrolysis using CP/MAS 13C NMR spectra in combination with line-fitting analysis.

Unit Definition

One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

substrate

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C9422-10KU:
C9422-VAR:
C9422-5KU:
C9422-BULK:
C9422-2.5KU:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Does Product C9422, Cellulase from Trichoderma viride, contain any sugars as a stabilizer?

    Product C9422, Cellulase from Trichoderma viride,contains 10 - 20% dextrin as a stabilizer.

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    Ask a Scientist here.

Jack P Wang et al.
Nature communications, 9(1), 1579-1579 (2018-04-22)
A multi-omics quantitative integrative analysis of lignin biosynthesis can advance the strategic engineering of wood for timber, pulp, and biofuels. Lignin is polymerized from three monomers (monolignols) produced by a grid-like pathway. The pathway in wood formation of Populus trichocarpa
Eliana N Fissore et al.
Journal of agricultural and food chemistry, 58(6), 3793-3800 (2010-02-25)
Chemical and rheological characteristics of fractions enriched in soluble dietary fiber are reported. These fractions were obtained through acid hydrolysis of butternut (Cucurbita moschata Duch ex Poiret) and red beet (Beta vulgaris L. var. conditiva) cell wall enriched powders. Hydrolysis
Yunqiao Pu et al.
Carbohydrate research, 341(5), 591-597 (2006-01-31)
Fully bleached softwood kraft pulps were hydrolyzed with cellulase (1,4-(1,3:1,4)-beta-D-glucan 4-glucano-hydrolase, EC 3.2.1.4) from Trichoderma reesei. Supra-molecular structural features of cellulose during enzymatic hydrolysis were examined by using CP/MAS 13C NMR spectra in combination with line-fitting analysis. Different types of
Orsolya Erzsébet Szabó et al.
Carbohydrate polymers, 98(2), 1483-1489 (2013-09-24)
A commercial acidic cellulase enzyme complex was chosen in order to gain detailed information about the effect of low-frequency ultrasound (horn at 40 kHz) on the enzyme activity. The performance of the enzyme under sonication was also evaluated in a
Akihiko Nakamura et al.
Journal of synchrotron radiation, 20(Pt 6), 859-863 (2013-10-15)
Neutron protein crystallography (NPC) is a powerful tool for determining the hydrogen position and water orientation in proteins, but a much larger protein crystal is needed for NPC than for X-ray crystallography, and thus crystal preparation is a bottleneck. To
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