Skip to Content
Merck
All Photos(1)

Documents

529576

Sigma-Aldrich

PP2

≥95% (HPLC), liquid, Src protein tyrosine kinase inhibitor, Calbiochem®

Synonym(s):

InSolution PP2

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C15H16ClN5
Molecular Weight:
301.77
UNSPSC Code:
12352200
NACRES:
NA.77

product name

PP2, InSolution, ≥95%, 10 mM, reversible ATP-competitive inhibitor of the Src family of protein tyrosine kinases

Quality Level

100

Assay

≥95% (HPLC)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

clear colorless

shipped in

wet ice

storage temp.

−20°C

General description

A potent and selective inhibitor of the src family of tyrosine kinases. Its action is similar to PP1. Selectively inhibits p56lck (IC50 = 4 nM), p59fynT (IC50 = 5 nM), and Hck (IC50 = 5 nM) compared to other tyrosine kinases, such as EGF-R (IC50 = 480 nM), JAK2 (IC50 >50 µM) or ZAP-70 (IC50 >100 µM). Also potently inhibits anti-CD3-stimulated tyrosine phosphorylation of human T cells (IC50 = 600 nM).

Biochem/physiol Actions

Cell permeable: no
Primary Target
p56lck
Product competes with ATP.
Reversible: yes
Target IC50: 4 nM, 5 nM, 5 nM, 100 nM, against p56lck, p59fynT, Hck , and Src, respectively; 600 nM against anti-CD3-stimulated tyrosine phosphorylation of human T cells

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Physical form

A 10 mM (1 mg/331 µl) solution of PP2 (Cat. No. 529573) in DMSO.

Reconstitution

Following initial thaw, aliquot, purge with inert gas, and freeze (-20°C).

Other Notes

Hanke, J.H., et al. 1996. J. Biol. Chem.271, 695.



Selected Citations
Lee, J., et al. 2009. Cell Stem Cell5, 76.


Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F - closed cup - (Dimethylsulfoxide)

Flash Point(C)

87 °C - closed cup - (Dimethylsulfoxide)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

ShengPeng Wang et al.
Blood, 140(3), 171-183 (2022-04-21)
The extravasation of leukocytes is a critical step during inflammation that requires the localized opening of the endothelial barrier. This process is initiated by the close interaction of leukocytes with various adhesion molecules such as ICAM-1 on the surface of
Liam T Caven et al.
Frontiers in cellular and infection microbiology, 13, 1098420-1098420 (2023-03-17)
The obligate intracellular pathogen Chlamydia trachomatis is the causative agent of the most common bacterial sexually transmitted disease worldwide. While the host response to infection by this pathogen has been well characterized, it remains unclear to what extent host gene
Adèle Kerjouan et al.
Journal of cell science, 134(2) (2021-01-27)
Upon activation by different transmembrane receptors, the same signaling protein can induce distinct cellular responses. A way to decipher the mechanisms of such pleiotropic signaling activity is to directly manipulate the decision-making activity that supports the selection between distinct cellular
Ryann E Shor et al.
Molecular oncology, 16(1), 130-147 (2021-06-01)
Dormant, disseminated tumor cells (DTCs) are thought to be the source of breast cancer metastases several years or even decades after initial treatment. To date, a selective therapy that leads to their elimination has not been discovered. While dormant DTCs

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service