ZRB2069

Anti-XLF/NHEJ1 clone 1C16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinonimo/i: Anti-CXCL12/SDF1 Antibody, clone 5I2 ZooMAb^® Rabbit Monoclonal, Non-homologous end-joining factor 1, Protein cernunnos, XRCC4-like factor
• Codice UNSPSC: 12352203
• NACRES: NA.43

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 200
• Ricombinante: expressed in HEK 293 cells
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 1C16, recombinant monoclonal
• Descrizione: recombinant, expressed in HEK 293 cells
• Nome Commerciale: ZooMAb^® learn more
• Forma fisica: lyophilized
• PM: calculated mol wt 33.34 kDa; observed mol wt ~38 kDa
• Purificato mediante: using Protein A
• Reattività contro le specie: human
• Confezionamento: antibody small pack of 25
• Caratteristiche più verdi: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Convalida avanzata: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• tecniche: affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG
• Sequenza dell’epitopo: C-terminal
• N° accesso ID proteina: NP_001364427
• N° accesso UniProt: Q9H9Q4
• Categoria alternativa più verde: Aligned
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• Informazioni sul gene: human ... NHEJ1(79840)

Descrizione

Descrizione generale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificità

Clone 1C16 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Non-homologous end-joining factor 1 (XLF/NHEJ1). It targets an epitope within 21 amino acids from the C-terminal region.

Immunogeno

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Non-homologous end-joining factor 1 (XLF/NHEJ1).

Applicazioni

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected XLF/NHEJ1 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected XLF/NHEJ1 in Ramos cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound XLF/NHEJ1 peptide with a KD of 1.1 x 10-7 in an affinity binding assay.

Flow Cytometry Analysis: 1 µg from a representative lot detected XLF/NHEJ1 in one million Ramos cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected XLF/NHEJ1 in HeLa cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected XLF/NHEJ1 in Human prostate tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrizione del bersaglio

Non-homologous end-joining factor 1 (UniProt: Q9H9Q4; also known as Protein cernunnos, XRCC4-like factor) is encoded by the NHEJ1 (also known as XLF) gene (Gene ID: 79840) in human. XLF is a ubiquitously expressed DNA repair protein that is involved in non-homologous end joining (NHEJ) and is shown to be essential for double-strand break (DSB) repairs and V(D)J recombination. Together with PAXX, it collaborates with DNA polymerase lambda to promote joining of non-cohesive DNA ends. It may also act in concert with Ku to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are non-complementary or partially complementary. The XRCC4-XLF subcomplex is reported to bind to the DNA fragments of a DSB in a highly diffusive manner and strongly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other. The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factor

Stato fisico

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Ricostituzione

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Note legali

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable