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Key Documents

SRP5068

Sigma-Aldrich

PKN2/PRK2, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sinonimo/i:

MGC150606, MGC71074, PAK2, PKN2, PRK2, PRKCL2, PRO2042, Pak-2

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

Ricombinante

expressed in baculovirus infected Sf9 cells

Nome Commerciale

PRECISIO® Kinase

Saggio

≥70% (SDS-PAGE)

Forma fisica

buffered aqueous glycerol solution

Attività specifica

63-85 nmol/min·mg

PM

~145 kDa

N° accesso NCBI

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Informazioni sul gene

human ... PKN2(5586)

Descrizione generale

PKN2 (also known as PRK2) is a Rho effector and a member of the protein kinase C superfamily of serine/threonine kinases. PKN2 is an essential regulator of both entry into mitosis and exit from cytokinesis in HeLa S3 cells. PKN2 is required for abscission of the midbody at the end of the cell division cycle and for phosphorylation and activation of Cdc25B, the phosphatase required for activation of mitotic cyclin/Cdk1 complexes at the G2/M transition. C-terminus of PKN2 could be a potential drug target for effector-specific pharmacological intervention of Rho-medicated biological processes.

Stato fisico

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Nota sulla preparazione

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Note legali

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1


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Wee Guan Lim et al.
Archives of biochemistry and biophysics, 479(2), 170-178 (2008-10-07)
PRK2/PKNgamma is a Rho effector and a member of the protein kinase C superfamily of serine/threonine kinases. Here, we explore the structure-function relationship between various motifs in the C-terminal half of PRK2 and its kinase activity and regulation. We report
Anja Schmidt et al.
The EMBO journal, 26(6), 1624-1636 (2007-03-03)
Rho GTPases regulate multiple signal transduction pathways that influence many aspects of cell behaviour, including migration, morphology, polarity and cell cycle. Through their ability to control the assembly and organization of the actin and microtubule cytoskeletons, Rho and Cdc42 make

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