SRP4015

CD40Ligand/TRAP from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Sinonimo/i: CD154 antigen, CD40-L, T cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: mouse
• Ricombinante: expressed in E. coli
• Saggio: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Stato: lyophilized
• Potenza: <0.1 μg/mL ED₅₀
• PM: ~16.4 kDa
• Confezionamento: pkg of 25 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• Impurezze: endotoxin, tested
• N° accesso NCBI: P27548
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: mouse ... Cd40lg(21947)

Descrizione

Descrizione generale

CD40 Ligand (CD40L/TRAP) is a type II transmembrane glycoprotein belonging to the TNF superfamily of ligands. Natually, CD40L is a membrane-bound protein, but proteolytically cleaved 15-18 kDa soluble forms of CD40L are also fully active. CD40L plays an important role in the proliferation and differentiation of B-cells, and is critical for immunoglobulin (Ig) class switching. Recombinant mouse CD40 Ligand is a soluble 18 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CDE40L.

Stato fisico

Sterile filtered and then lyophilized from 10 mM Sodium Phosphate, pH 7.5 plus 0.1 M Arginine.

Ricostituzione

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.