SRP3074
IL-12p40 human
recombinant, expressed in CHO cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
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About This Item
Codice UNSPSC:
12352202
NACRES:
NA.32
Origine biologica
human
Ricombinante
expressed in CHO cells
Saggio
≥98% (HPLC)
≥98% (SDS-PAGE)
Forma fisica
lyophilized
PM
40 kDa
Confezionamento
pkg of 10 μg
tecniche
cell culture | mammalian: suitable
Impurezze
<0.1 EU/μg endotoxin, tested
Colore
white to off-white
N° accesso UniProt
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... IL12B(3593)
Descrizione generale
IL-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23. Recombinant human IL-12 p40 is a 40.0 kDa protein containing 306 amino acid residues.
The interleukin-12 subunit β (IL12β) gene is localized on human chromosome 5q31–33. The encoded protein is part of IL-12, which is a heterodimer. It is mainly expressed by antigen-presenting cells (APCs).
Azioni biochim/fisiol
IL-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Recombinant human IL-12 p40 is a 40.0 kDa protein containing 306 amino acid residues.
Interleukin-12 subunit β (IL12β) is an immunoregulatory cytokine. It is involved in pathogenic defense responses and is also suggested as an anticancer therapeutic agent. IL12B is responsible for polarizing naive CD4+ cells toward a TH1 (T helper) phenotype and inducing IFN-γ (interferon) secretion by T and natural killer (NK) cells. This protein also stimulates the differentiation of cytotoxic CD8+ cells and enhances the activity of NK cells. IL12B is linked with many autoimmune diseases, such as psoriasis, systemic lupus erythematosus, Crohn′s disease and ulcerative colitis.
Sequenza
p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
Stato fisico
Lyophilized from 1x PBS, pH 7.2.
Ricostituzione
Centrifuge the vial prior to opening. Reconstitute in 1x PBS, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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PloS one, 10(6), e0130982-e0130982 (2015-06-24)
This study aims to determine whether the genetic polymorphisms of IL-12B gene is a susceptibility factor to Ankylosing spondylitis (AS) in mainland Han Chinese population. Eight single-nucleotide polymorphisms (SNPs) (rs10045431, rs11167764, rs3212227, rs6556412, rs6556416, rs6871626, rs6887695 and rs7709212) in the
M K Gately et al.
Journal of immunology (Baltimore, Md. : 1950), 147(3), 874-882 (1991-08-01)
IL-12 is a heterodimeric cytokine that was identified on the basis of its ability to synergize with IL-2 in the induction of cytotoxic effector cells and was originally called cytotoxic lymphocyte maturation factor (CLMF). IL-12 was also found to stimulate
M B Atkins et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 3(3), 409-417 (1997-03-01)
A Phase I dose escalation trial of i.v. administered recombinant human interleukin 12 (rhIL-12) was performed to determine its toxicity, maximum tolerated dose (MTD), pharmacokinetics, and biological and potential antineoplastic effects. Cohorts of four to six patients with advanced cancer
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