SRP2082

WT-1 (+KTS) human

recombinant, expressed in insect cells, ≥60% (SDS-PAGE)

• Codice UNSPSC: 12352200
• NACRES: NA.26

Proprietà

• Origine biologica: human
• Ricombinante: expressed in insect cells
• Saggio: ≥60% (SDS-PAGE)
• Stato: frozen liquid
• Confezionamento: pkg of 5 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• Concentrazione: 150 μg/mL
• Colore: clear colorless
• N° accesso NCBI: NM_024425
• N° accesso UniProt: P19544
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... WT1(7490)

Descrizione

Azioni biochim/fisiol

WT-1, the product of Wilms′ tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT1 protein. Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms′ tumor. With the inclusion of exon 5, WT1 (KTS+) binds to both DNA and RNA and is RNase but not DNase sensitive. This form also co-localizes with splicing factors in a speckled nuclear particle, suggesting that the WT1 protein may function as both a transcription factor and a splicing regulator.

Stato fisico

Clear and colorless frozen liquid solution

Nota sulla preparazione

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable