SAB4702058

Monoclonal Anti-CD45 APC-Cyanine7 produced in mouse

• Sinonimo/i: B220, GP180, LCA, LY5, T200, TPC
• Codice UNSPSC: 12352203

Proprietà

• Coniugato: APC-Cy7 conjugate
• Livello qualitativo: 100
• Tipo di anticorpo: primary antibodies
• Clone: MEM-28, monoclonal
• Forma fisica: buffered aqueous solution
• Reattività contro le specie: human
• tecniche: flow cytometry: suitable
• Isotipo: IgG1
• N° accesso UniProt: P08575
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... PTPRC(5788)

Descrizione

Descrizione generale

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

Specificità

The antibody MEM-28 reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.

Immunogeno

Human thymocytes and T lymphocytes.

Stato fisico

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Nota sulla preparazione

Purified antibody is conjugated with activated tandem dye of allophycocyanin-cyanine 7 (APC-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Stoccaggio e stabilità

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Altre note

The antibody solution should be gently mixed before use.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable