SAB4200284

Anti-Neurocalcin-δ antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody

• Sinonimo/i: Anti-NCALD
• Codice UNSPSC: 12352203
• NACRES: NA.44

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Stato: buffered aqueous solution
• PM: antigen ~22 kDa
• Reattività contro le specie: human, mouse, rat
• Convalida avanzata: recombinant expression; Learn more about Antibody Enhanced Validation
• Concentrazione: ~0.5 mg/mL
• tecniche: western blot: 0.5-1.0 μg/mL using mouse brain extract (S1 fraction) and lysates of HEK-293T cells over-expressing human neurocalcin-δ.
• N° accesso UniProt: P61601
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... NCALD(83988) ; mouse ... Ncald(52589) ; rat ... Ncald(553106)

Descrizione

Descrizione generale

The neurocalcin-δ (NCALD) gene is mapped to human chromosome 8q22.3. The encoded protein belongs to a large family of neuronal calcium (Ca²⁺) sensor (NCS) proteins and is a member of visinin-like protein (VILIP) subfamily. Neurocalcin-δ is also one of the EF-hand-containing Ca²⁺-binding proteins. It is predominantly expressed in the Purkinje cells of the cerebellum and is also present in the olfactory epithelium.

Specificità

Anti-Neurocalcin-d specifically recognizes human and mouse neurocalcin-d.

Immunogeno

synthetic peptide corresponding to a sequence located near the N-terminus of human neurocalcin-δ, conjugated to KLH. The corresponding sequence is identical in mouse and rat neurocalcin-δ.

Applicazioni

Anti-Neurocalcin-δ antibody produced in rabbit is suitable for immunoblotting.

Azioni biochim/fisiol

Neurocalcin-δ (VILIP-3) is a neuronal calcium (Ca²⁺) sensor. It binds cooperatively to three Ca²⁺ ions. Neurocalcin-δ influences cyclic guanosine monophosphate (cGMP) signaling in the retina, by acting as a regulator of the retinal guanylate cyclase (retGC1)/ photoreceptor membrane guanylyl cyclase 1 (ROSGC1). Neurocalcin-δ interacts with retGC1 in a calcium (Ca2+) dependent manner. It colocalizes with retGC1 in neurons in the inner plexiform layer of the retina, in amacrine and ganglion cells where it may influence synaptic signaling processes. Neurocalcin-δ has been shown to interact with actin, clathrin, and α- and β-tubulins, indicating that it may be involved with clathrin-coated vesicle traffic. It interacts and activates the olfactory guanylate cyclase ONE-GC, suggesting that it may function in regulating the odorant signaling pathway.

Stato fisico

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 1% BSA as stabilizer and 15 mM sodium azide.

Stoccaggio e stabilità

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable