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S8015

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

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About This Item

Codice UNSPSC:
41105319
NACRES:
NA.25

Grado

for molecular biology

Forma fisica

powder blend

pH

6.9-7.1 (20-25 °C, 20 ×)

Attività estranea

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

Temperatura di conservazione

room temp

Descrizione generale

SSC (Saline Sodium Citrate) Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization. SSC buffer powder should be reconstituted to the final indicated volume using molecular biology grade water.

Applicazioni

Suitable for use in washing buffer for Northern and Southern hybridization procedures.

Caratteristiche e vantaggi

  • Easy to reconstitute powder
  • Multi-purpose use in hybridizations

Componenti

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

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Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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