PP0530
GlycoProfile™ 2-AA Labeling Kit
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Informazioni su questo articolo
UNSPSC Code:
12352200
EC Number:
204-287-5
NACRES:
NA.32
General description
The GlycoProfile™ labeling kits are designed for efficient labeling of N-linked, O-linked, and glycosylphosphatidylinositol (GPI) anchored glycans with either 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA). These small fluorophores increase the spectral absorption of glycans, improving the detection of labeled glycans by HPAE (high-performance anion exchange chromatography), HPLC, and ESI-MS (electrospray mass spectroscopy) methods. Binding is robust and the resulting dye-glycan conjugates are stable, with no degradation during post-labeling analysis.
Application
While 2-AB is slightly less sensitive than 2-AA, it is suitable for downstream glycan analysis by HPAE, HPLC and ESI-MS. Although suitable for the same applications, 2-AA is better suited for SDS-PAGE.
GlycoProfile™ 2-AA Labeling Kit has been used in the labeling of fluorescently derivatized N-glycans from chinese hamster ovary CHO cells.
Legal Information
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC
Solo come componenti del kit
N° Catalogo
Descrizione
- Anthranilic acid (2-AA) 2 x 6
- Dimethyl sulfoxide (DMSO) 2 x 350
- Acetic acid, glacial 2 x 200
- Reductant (sodium cyanoborohydride) 2 x 6
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 1
flash_point_f
302.0 °F - closed cup
flash_point_c
150 °C - closed cup
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