PLA0201

Rabbit anti-14-3-3 Sigma Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

• Sinonimo/i: 14-3-3 sigma, Stratifin, YWHAS, epithelial cell marker protein 1
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Grado: Powered by Bethyl Laboratories, Inc.
• Reattività contro le specie: human
• tecniche: immunohistochemistry: 1:2,000-1:10,000; immunoprecipitation (IP): 2-5 μg/mg; western blot: 1:2,000- 1:10,000
• Numero d’accesso: NP_006133.1
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SFN(2810)

Descrizione

Descrizione generale

Rabbit anti-14-3-3 Sigma or stratifin is mapped to human chromosome 1p36.11. 14-3-3σ protein is expressed as seven isoforms and has molecular weight in the range of 25 to 30 kDa. It is an acidic homodimeric protein and harbors pleckstrin homology (PH) domain. 14-3-3σ is highly expressed in epidermis.

Immunogeno

The epitope recognized by PLA0201 maps to a region between residue 198 and 248 of human 14-3-3 sigma using the numbering given in entry NP_006133.1 (GeneID 2810).

Applicazioni

Rabbit anti-14-3-3 Sigma Antibody, Affinity Purified has been used in the pull down assay for Rho associated coiled-coil containing protein kinase 1 (ROCK1) proteins and heat shock 70 kDa 4 protein (Hsp74) in HCT116 cancer cells.

Azioni biochim/fisiol

Rabbit anti-14-3-3 or stratifin participates in several biological events including apoptosis and cell differentiation. It regulates the transcription matrix metallopeptidases (MMPs) in fibroblasts and may serve as therapeutic potential in wound healing. 14-3-3σ is less expressed and is hyper methylated in breast cancer cells. It plays a key role in linking signalling proteins and in the enhancing of protein kinase C functionality. A mutation in the stratifin gene is implicated in epilation phenotype in mice. It interacts with ubiquitin-specific protease 8 (USP8) and is highly expressed in human lung adenocarcinoma.

Stato fisico

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Altre note

14-3-3 sigma was identified as an epithelial cell marker. It is part of the 14-3-3 family of proteins in which seven isoforms have been identified: beta, zeta, gamma, eta, epsilon, tau, and sigma. 14-3-3 proteins function as adaptors that bind with a number of partners to mediate various signaling pathways. 14-3-3 sigma is also called stratifin or HME1 and appears to function as a tumor suppressor whose expression can be downregulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable