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P5459

Sigma-Aldrich

Proteasi

glycerol solution (50%)

Sinonimo/i:

Proteinasi, Subtilisina A

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About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero MDL:
Codice UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54

Origine biologica

Bacillus licheniformis

Forma fisica

glycerol solution (50%)

Attività specifica

≥7 units/mg protein (biuret)

Concentrazione

≥50 mg/mL

Solubilità

0.85% sodium chloride: soluble, clear (Diluted)
H2O: miscible

Temperatura di conservazione

2-8°C

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Categorie correlate

Descrizione generale

The enzyme hydrolyzes gelatin optimally at 50 °C over the range of pH 8-10. It is stable between pH 6-10 at 50 °C. Commercially, it is used in laundry detergent formulations and also for the recovery of silver from photographic emulsions.

Applicazioni

Protease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Bacillus licheniformis, product P5459, has been used to hydrolyze muscle during the isolation of mitochondria from skeletal muscle.

Azioni biochim/fisiol

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

Confezionamento

Package size based on protein content.

Definizione di unità

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Pittogrammi

Health hazardCorrosion

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Certificati d'analisi (COA)

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