Passa al contenuto
Merck
Tutte le immagini(1)

Documenti

L2011

Sigma-Aldrich

D-Lactic Dehydrogenase from Lactobacillus leichmannii

ammonium sulfate suspension, ≥250 units/mg protein (biuret)

Sinonimo/i:

Lactate, (R)-Lactate:NAD+ oxidoreductase, D−LDH

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Numero CAS:
9028-36-8
Classificazione EC (Enzyme Commission):
1.1.1.28 (BRENDA, IUBMB)
Numero MDL:
MFCD00130907
Codice UNSPSC:
12352204
NACRES:
NA.54

Forma fisica

ammonium sulfate suspension

Livello qualitativo

200

Attività specifica

≥250 units/mg protein (biuret)

Attività estranea

Malic dehydrogenase <0.5% of base activity

Temperatura di conservazione

2-8°C

Descrizione generale

Research area: Cell Signalling D-lactate dehydrogenase belongs to the D-isomer-specific 2-hydroxyacid dehydrogenase family. Lactate dehydrogenase (LD) is a tetramer consisting of two (H and M) subunits. It has five isozymes (LD1 to LD5) and this composition varies in different tissues. LD1 is abundant in the kidney, heart, and erythrocytes. LD5 is found in the liver and skeletal muscles. LD is a ubiquitous molecule found in plants, yeast, mammals, and microorganisms and is a member of the oxidoreductase family.

Azioni biochim/fisiol

Lactic dehydrogenase (LDH)plays an important role in the glycolytic pathway where it converts pyruvate tolactate by using NAD+ as a co-factor. It is considered animportant molecule that can be used in cancer therapy as it acts as aglycolytic inhibitor. Inhibition of LDH is associated with blocking of aerobicglycolysis in tumour cells.

Definizione di unità

One unit will reduce 1.0 μmole of pyruvate to D-lactate per min at pH 7.0 at 25 °C.

Stato fisico

Suspension in 3.2 M (NH4)2SO4, pH 6.0

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

H Taguchi et al.
The Journal of biological chemistry, 266(19), 12588-12594 (1991-07-05)
The gene encoding D-lactate dehydrogenase (D-lactate: NAD+ oxidoreductase, EC 1.1.1.28) of Lactobacillus plantarum has been sequenced, and expressed in Escherichia coli cells with an inducible expression plasmid, in which the 5'-noncoding region of the gene was replaced with the tac
Torben Larsen
Analytical biochemistry, 539, 152-157 (2017-11-06)
D-lactic acid in the mammalian body is mainly of microbiological origin and is often located somewhere along the digestive tract. Surgical, extensive re-sectioning of the small bowel may be one of the risk factors for altered balance in the microbiological
D L Zhang et al.
Postgraduate medical journal, 79(928), 110-112 (2003-03-04)
A 12 year old boy presented with 11 episodes of weakness, ataxia, nausea, slurred speech, dehydration, and sometimes severe lethargy bordering on coma. A year previously the boy had small intestinal resections leaving 20 cm of small bowel remaining. D-lactic
Monica Gallo et al.
Frontiers in bioscience (Landmark edition), 20(8), 1234-1249 (2015-05-12)
Despite the intense scientific efforts made, there are still many tumors that are difficult to treat and the percentage of patient survival in the long-term is still too low. Thus, new approaches to the treatment of cancer are needed. Cancer
Clinical utility of lactate dehydrogenase: a historical perspective.
Ishwarlal Jialal et al.
American journal of clinical pathology, 143(2), 158-159 (2015-01-18)
Visualizza tutti i documenti correlati

Articoli

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.