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G5922

Sigma-Aldrich

Anti-GW182 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinonimo/i:

Anti-TNRC6A, Anti-Trinucleotide repeat containing 6A

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Coniugato

unconjugated

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

human

Concentrazione

~1.0 mg/mL

tecniche

indirect immunofluorescence: 2.5-5.0 μg/mL using human epithelial HEp-2 cells

N° accesso UniProt

Q8NDV7

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

mouse ... Tnrc6a(233833)
rat ... Tnrc6a(308971)

Descrizione generale

GW182 is a phosphorylated cytoplasmic autoantigen. It contains multiple glycine-tryptophan (GW) repeats along with a single RNA recognition motif. GW182 is a protein of 182 kDa, found in mammalian cell types.

Applicazioni

Anti-GW182 antibody produced in rabbit has been used as a primary antibody for immunostaining of HeLa cells and human hepatoma cells. It has also been used for indirect immunofluorescence at a working concentration of 2.5-5.0μg/mL using human epithelial HEp-2 cells.

Azioni biochim/fisiol

Glycine-tryptophan (GW) bodies or P-bodies are cytoplasmic bodies that are involved in mRNA degradation, storage and translational repression. These bodies contain Argonaute proteins and m-RNAs that associate with GW182. GW182 is involved in post-transcriptional gene silencing/RNA interference (RNAi) via the microRNA pathway. Inhibition of gene expression leads to destruction of GWBs and disruption of RNAi and microRNA-induced gene silencing as it forms an integral component of GWBs.

Stato fisico

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

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GW182 is critical for the stability of GW bodies expressed during the cell cycle and cell proliferation
Yang Z, et al.
Journal of Cell Science, 117(23), 5567-5578 (2004)
A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles
Eystathioy T, et al.
Molecular Biology of the Cell, 13(4), 1338-1351 (2002)
Andrew Jakymiw et al.
Journal of cell science, 120(Pt 8), 1317-1323 (2007-04-03)
GW bodies, also known as mammalian P-bodies, are cytoplasmic foci involved in the post-transcriptional regulation of eukaryotic gene expression. Recently, GW bodies have been linked to RNA interference and demonstrated to be important for short-interfering-RNA- and microRNA-mediated mRNA decay and
Terence N Bukong et al.
Hepatology (Baltimore, Md.), 57(1), 70-80 (2012-08-18)
Alcohol use and hepatitis C virus (HCV) infection synergize to cause liver damage, and microRNA-122 (miR-122) appears to play a key role in this process. Argonaute 2 (Ago2), a key component of the RNA-induced silencing complex (RISC), has been shown
Jidong Liu et al.
Nature cell biology, 7(12), 1261-1266 (2005-11-15)
In animals, the majority of microRNAs regulate gene expression through the RNA interference (RNAi) machinery without inducing small-interfering RNA (siRNA)-directed mRNA cleavage. Thus, the mechanisms by which microRNAs repress their targets have remained elusive. Recently, Argonaute proteins, which are key
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