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F4890

Monoclonal Anti-Rabbit Immunoglobulins–FITC antibody produced in mouse

clone RG-16, purified immunoglobulin, buffered aqueous solution

Sinonimo/i:

Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)

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0.2 mL
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219,00 €
186,15 €

Informazioni su questo articolo

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
FITC conjugate
Clone:
RG-16, monoclonal
Application:
IF, IHC (p)
Citations:
17

186,15 €

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biological source

mouse

Quality Segment

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

RG-16, monoclonal

form

buffered aqueous solution

should not react with

human, pig, chicken, bovine, horse, guinea pig, turkey, rat, canine, goat, feline, sheep

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, indirect immunofluorescence: 1:50 using using chicken fibroblast cells and primary antibody produced in rabbit

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

Application

Monoclonal Anti-Rabbit Immunoglobulins-FITC antibody produced in mouse has been used in:
  • immunocytochemistry
  • immunohistochemistry
  • immunofluorescence

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

Biochem/physiol Actions

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

Physical form

Solution in 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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B3275B5283R1008
description

clone RG-16, purified immunoglobulin, buffered aqueous solution

description

clone RG-16, purified immunoglobulin, buffered aqueous solution

description

clone RG-96, purified from hybridoma cell culture

description

clone RG-96, ascites fluid

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

ascites fluid

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, indirect immunofluorescence: 1:50 using using chicken fibroblast cells and primary antibody produced in rabbit

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500, western blot: 1:200,000, western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well), direct ELISA: 1:60,000

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000, western blot (chemiluminescent): 1:200,000-1:400,000, direct ELISA: 1:150,000

technique(s)

western blot: suitable, dot blot: suitable, immunohistochemistry (frozen sections): suitable, indirect ELISA: 1:2,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

conjugate

FITC conjugate

conjugate

biotin conjugate

conjugate

biotin conjugate

conjugate

unconjugated

isotype

IgG1

isotype

IgG1

isotype

IgG1

isotype

IgG1

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C


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Classe di stoccaggio

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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