F3022

Anti-β-actina monoclonale

clone AC-15, purified from hybridoma cell culture

• Sinonimo/i: Monoclonal Anti-β-Actin
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: FITC conjugate
• Clone: AC-15, monoclonal
• Application: IF (d)
• Citations: 23

Proprietà

• biological source: mouse
• Quality Segment: 200
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: AC-15, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 42 kDa
• species reactivity: sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
• should not react with: Dictyostelium discoideum
• technique(s): direct immunofluorescence: 1:250 using cultured human and chicken fibroblasts
• isotype: IgG1
• UniProt accession no.: P60709
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ACTB(60) ; mouse ... Actb(11461) ; rat ... Actb(81822)

Descrizione

General description

Nella colorazione delle criosezioni di tessuti ultrasottili di ventrigli di pollo, l′anticorpo marca i corpi densi, i canali longitudinali che collegano i corpi densi consecutivi che sono occupati anche dalla desmina, e la placca densa associata alla membrana. Non colora i muscoli cardiaci e scheletrici adulti, tranne che per alcune tracce che sono dovute alla contaminazione del campione con cellule non muscolari o che possono comparire se si utilizzano tessuti embrionali.

Monoclonal Anti-b-Actin (mouse IgG1 isotype) is derived from the AC-15 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. ACTB (actin beta) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1. Actin is a eukaryotic protein. It belongs to the major cytoskeletal protein family.

Immunogen

peptide N-terminale lievemente modificato della β-actina citoplasmatica, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, coniugato a KLH.

Application

L′anti-actina murina monoclonale è stata utilizzata come controllo di caricamento per l′analisi con metodica Western blot di lisati di proteine epatiche di ratto.

L′anticorpo anti-actina murino monoclonale è stato utilizzato come controllo di caricamento per l′analisi con metodica Western blot di proteine immunoprecipitate da co-colture di gangli della radice dorsale di ratto.

Anti-β-Actin-FITC antibody, Mouse monoclonal has been used in:

• western blotting
• immunohistochemistry
• flow cytometry
• direct immunofluorescent staining
• dual immuno-fluorescent staining

Biochem/physiol Actions

β-Actin modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.

Actins are majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.

Actins is a eukaryotic protein. It belongs to the major cytoskeletal protein family and majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Classe di stoccaggio: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)