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E8156

Sigma-Aldrich

Anti-eRF1

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinonimo/i:

Anti-Eukaryotic translation termination factor 1, Anti-RF1, Anti-Release factor 1, Anti-eTF1

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Coniugato

unconjugated

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

PM

antigen 50 kDa

Reattività contro le specie

rat, human, mouse

Confezionamento

antibody small pack of 25 μL

Concentrazione

~1 mg/mL

tecniche

immunoprecipitation (IP): 1-2 μg using HeLa cell lysates.
western blot: 0.5-1 μg/mL using 3T3 or Rat1 cell extracts.

N° accesso UniProt

P62495

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... ETF1(2107)
mouse ... Etf1(225363)
rat ... Etf1(307503)

Categorie correlate

Descrizione generale

Eukaryotic translation termination factor 1 (eRF1) is a protein encoded by the ETF1 gene in humans. It belongs to eRF1 family and the amino-acid sequence is highly conserved.

Applicazioni

Anti-eRF1 antibody produced in rabbit has been used in western blotting and immunoprecipitation.

Azioni biochim/fisiol

Eukaryotic translation termination factor 1 (eRF1) proteins are directly implicated in the termination of translation in eukaryotes. It plays an important role in the termination of protein synthesis, which is performed by three domains (N, M and C) of eRF1. Rearrangement in eRF1 leads to N-domain accommodation into the ribosomal A site.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

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Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division
Petsch K, et al.
Plant Physiology, 139(1), 115-126 (2005)
Identification and characterization of a novel ubiquitous nucleolar protein `NARR?encoded by a gene overlapping the rab34 oncogene
Zougman A, et al.
Nucleic Acids Research, 39(16), 7103-7113 (2011)
L Frolova et al.
Nature, 372(6507), 701-703 (1994-12-15)
The termination of protein synthesis in ribosomes is governed by termination (stop) codons in messenger RNAs and by polypeptide chain release factors (RFs). Although the primary structure of prokaryotic RFs and yeast mitochrondrial RF is established, that of the only
Toshiaki Shigeoka et al.
Nucleic acids research, 40(14), 6887-6897 (2012-05-05)
Upf1 is a highly conserved RNA helicase essential for nonsense-mediated mRNA decay (NMD), an mRNA quality-control mechanism that degrades aberrant mRNAs harboring premature termination codons (PTCs). For the activation of NMD, UPF1 interacts first with a translation-terminating ribosome and then
Tatsuaki Kurosaki et al.
Nature structural & molecular biology, 25(10), 940-950 (2018-10-03)
Nonsense-mediated messenger RNA decay (NMD) controls mRNA quality and degrades physiologic mRNAs to fine-tune gene expression in changing developmental or environmental milieus. NMD requires that its targets are removed from the translating pool of mRNAs. Since the decay steps of
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