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D8417

4′,6-diamidino-2-fenilindolo

powder, BioReagent, suitable for cell culture, ≥98% (HPLC), suitable for fluorescence

4′,6-Diamidino-2-phenylindole dihydrochloride

Sinonimo/i:

2-(4-amidinofenil)-6-indolocarbammidina, DAPI

Autenticati per visualizzare i prezzi organizzativi e contrattuali.

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A voi/SKUDisponibilitàPrezzo
1 mg

Disponibile per la spedizione OGGIdaSchnelldorf Distribution

64,30 €
5 mg

Disponibile per la spedizione OGGIdaSchnelldorf Distribution

193,00 €
10 mg

Disponibile per la spedizione OGGIdaSchnelldorf Distribution

350,00 €

Informazioni su questo articolo

Formula empirica (notazione di Hill):
C16H15N5 · 2HCl
Numero CAS:
Peso molecolare:
350.25
UNSPSC Code:
41116130
NACRES:
NA.75
PubChem Substance ID:
EC Number:
249-186-7
Beilstein/REAXYS Number:
4894417
MDL number:

64,30 €


Disponibile per la spedizione OGGIDettagli


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product line

BioReagent

Quality Segment

assay

≥98% (HPLC)

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 20 mg/mL, PBS: insoluble

ε (extinction coefficient)

30 at 263 nm in H2O

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

General description

Heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.
4′,6-Diamidino-2-phenylindole dihydrochloride is a fluorescent dye for staining DNA. It strongly interacts with the dAT-rich (deoxy adenine-thymine) regions of the DNA.

Application

DAPI è parecchie volte più sensibile del bromuro di etidio per colorare il DNA in gel di agarosio.
DAPI è parecchie volte più sensibile del bromuro di etidio per colorare il DNA in gel di agarosio. Può essere impiegato per fotoimpronte del DNA, rivelare sonde ibridizzate in applicazioni blotting visualizzando specificamente il complesso a doppio filamento, studiare i cambiamenti nel DNA e analizzare con la citometria di flusso il contenuto di DNA durante l′apoptosi. La colorazione via DAPI si è dimostrata un metodo di rivelazione sensibile e specifico dei micoplasma.
4′,6-Diamidino-2-phenylindole dihydrochloride has been used in staining of various cells in immunocytochemistry and cytochemistry, human subcutaneous adipose tissues for fluorescence imaging, embryo sections for immunofluorescence.

Biochem/physiol Actions

Sonda fluorescente per DNA permeabile alle cellule che si attacca al solco minore. Si attacca al solco minore del DNA a doppio filamento (preferenzialmente a DNA ricco di T) a formare un complesso stabile con una fluorescenza circa 20 volte maggiore del DAPI da solo.

Disclaimer

Protect from light.

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Questo articolo
268298MBD0020D9542
DAPI for nucleic acid staining

Sigma-Aldrich

D9542

DAPI

suitability

suitable for fluorescence

suitability

-

suitability

suitable for nucleic acid staining

suitability

suitable for fluorescence

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable

technique(s)

transfection: suitable

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex ~359 nm, λem ~461 nm

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

solubility

H2O: 20 mg/mL, PBS: insoluble

solubility

water: 5 mg/mL, ethanol: soluble, methanol: soluble

solubility

-

solubility

H2O: 20 mg/mL, PBS: insoluble

form

powder

form

solid

form

liquid

form

powder

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

300


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pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

target_organs

Respiratory system

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Domande

1–2 di 2 domande  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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