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D5523

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium - low glucose

With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinonimo/i:

DME, DMEM

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About This Item

Numero MDL:
Codice UNSPSC:
12352207
NACRES:
NA.75

Livello qualitativo

Forma fisica

powder

tecniche

cell culture | mammalian: suitable

Componenti

L-glutamine: yes
NaHCO3: no
sodium pyruvate: yes
phenol red: yes
HEPES: no
glucose: low

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Categorie correlate

Descrizione generale

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME) that comprises a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types.

Applicazioni

Dulbecco′s Modified Eagle′s medium-low glucose has been used in the differentiation and culture of human and murine mesenchymal stem cells (MSCs).

Quantità

Formulated to contain 10.0 grams of powder per liter of medium.

Ricostituzione

Supplement with 3.7 g/L sodium bicarbonate.

Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2 - Skin Sens. 1

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Protocolli

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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