This product is a set of two tablets - one DAB tablet and one Urea Hydrogen Peroxide tablet. When prepared according to instructions, the solution should be used within one hour for best results.
D4293
3,3′-Diaminobenzidine
SIGMAFAST™, chromogenic, tablet
Sinonimo/i:
DAB tablets
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Scegli un formato
5 SETS
70,10 €
50 SETS
353,00 €
5 X 50 SET
942,00 €
Scegli un formato
Cambia visualizzazione
5 SETS
70,10 €
50 SETS
353,00 €
5 X 50 SET
942,00 €
About This Item
Nome del prodotto
SIGMAFAST™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 5 mL
Stato
tablet
Temperatura di conservazione
−20°C
Stringa SMILE
Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2
HSTOKWSFWGCZMH-UHFFFAOYSA-N
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Categorie correlate
Applicazioni
Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets
Ricostituzione
Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.
Note legali
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Articoli
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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Hello, your product D4293, SIGMAFAST™ 3,3′-Diaminobenzidine tablets, can be used for how many months after dissolving in 5ml of distilled water?
1 risposta-
Utile?
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Hello, Can https://www.sigmaaldrich.com/US/en/product/sigma/d4293?icid=sharepdp-clipboard-copy-productdetailpage this product be used to develop western blots? Thank you, Regards Vanda
1 risposta-
D4293, SIGMAFAST™ 3,3′-Diaminobenzidine tablets, can be used for WB on PVDF membranes, please see attached reference paper stating below: "The PVDF was then collected and rinsed 3 times for 5–10 min each wash with TBS. The blot was then developed appropriately with 3',3-Diaminobenzidine tablets for developing peroxidase (D4293 Sigma-Aldrich) and BCIP/NBT for alkaline phosphatase (B5655 Sigma-Aldrich) and the blot was imaged."Scientific Neurogenic marker expression in differentiating human adipose derived adult mesenchymal stem cells Neus Gomila Pelegri1,2, Bruce K. Milthorpe1 , Catherine A. Gorrie2 , Jerran Santos1. Stem Cell Investig 2023;10:7
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