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Key Documents

CWA-1032

Sigma-Aldrich

Anti-Mouse CD5 (53-7.3) ColorWheel® Dye-Ready mAb

greener alternative

for use with ColorWheel® Dyes (Required, sold separately)

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rat

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

53-7.3, monoclonal

Nome Commerciale

ColorWheel®

Forma fisica

lyophilized

PM

calculated mol wt 53.85 kDa

Reattività contro le specie

mouse

Confezionamento

antibody small pack of 25 μL

Caratteristiche più verdi

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

tecniche

flow cytometry: suitable

Isotipo

IgG2aκ

Sequenza dell’epitopo

Unknown

N° accesso ID proteina

N° accesso UniProt

Compatibilità

for use with ColorWheel® Dyes (Required, sold separately)

Categoria alternativa più verde

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

mouse ... CD5(12507)

Descrizione generale

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificità

Clone 53-7.3 is a rat monoclonal antibody that detects murine CD5. It binds to a monomorphic epitope on CD5.

Immunogeno

Murine thymus/spleen cells

Applicazioni

Quality Control Testing:

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: Staining of one million mouse splenocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1032, Anti-Mouse CD5 (53-7.3) ColorWheel® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Rat IgG2a isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibilità

Requires ColorWheel® Dye (Sold Separately)

Descrizione del bersaglio

T-cell surface glycoprotein CD5 (UniProt: P13379; also known as Lymphocyte antigen 1, Ly-1, Lyt-1, CD5) is encoded by the Cd5 (also known as Ly-1) gene (Gene ID: 12507) in murine species. CD5 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 24-371), a transmembrane domain (aa 372-401), and a cytoplasmic domain (aa 402-494). In its extracellular domain it contains three scavenger receptor cysteine-rich (SRCR) domains. It is expressed on the surface of T cells bearing the β heterodimer T cell receptor (TCR) and a subset of murine B cells known as B-1a cells. It is either not present or is present at low density on the T cell subset. CD5 serves as a transmembrane receptor that regulates T cell proliferation, functions, and development. CD72 is recognized as one of the ligands for CD5. It is considered as an activation marker of T cells. Its expression is reported to increase according to the magnitude of the signal delivered by the TCR. It has been reported that the absence of CD5 in mice can enhance signaling and activation of double positive thymocytes induced by TCR stimulation, indicating that it acts as a negative regulator of TCR signaling in T cells. In mature, peripheral T cells, CD5 is reported to be present in lipid rafts of the T cell surface, where it promotes CD3 redistribution into rafts and upregulates ZAP-70 and LAT activation. (Ref.: Voisinne, G., et al. (2018). Front. Immunol. 9; 2900; Gary-Gouy, H., et al. (1997). J. Immunol. 159(8); 3739-3747).

Stato fisico

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Ricostituzione

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Note legali

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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