DCH-012 human cell line

IHW Number: 9187, 94082287

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human blood
• Etnia: Thai
• Genere: male
• Modalità di accrescimento: Suspension
• Cariotipo: Not specified
• Morfologia: Lymphoblastoid
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice

Descrizione

Descrizione generale

Consanguineous: No

Homozygous: No

Descrizione della linea cellulare

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Profilo DNA

A: 68 33
B: 08 46
HLA DRB1: 0301 09
HLA DRB3: 202
HLA DRB4: 101
HLA DQAP: 3.2 4.1
HLA DQA1: 0501 0302
HLA DQB1: 0201 0303
HLA DPB1: 0501 1701

Profilo sierologico

HLA A: 28 33
HLA A ief:
HLA C: 1 7
HLA B: 8 46

Terreno di coltura

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Mantenimento delle subcolture

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Esclusione di responsabilità

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.