IgH-2

90030804, lizard cardiovascular , Epithelial-like

• Codice UNSPSC: 41106514

Proprietà

• product name: IgH-2, 90030804
• Origine biologica: lizard cardiovascular
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 34
• Morfologia: Epithelial-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Iguana heart

Descrizione della linea cellulare

From the heart of a normal immature male Iguana. Cells can support the replication of herpes simplex, PRV, vaccinia and Iguana viruses at 36°C. The only reported reptilian line to be established and serially propagated at an incubation temperature as high as 36°C.

Applicazioni

Virus replication studies: HSV, PRV, vaccinia and Iguana viruses

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 36°C.

Altre note

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